Figure 15. Clock/Bmal1 suppresses GR-induced transcriptional activity through acetylation. Clock physically interacts with GR LBD through its nuclear receptor-interacting domain and suppresses GR-induced transcriptional activity by acetylating with its intrinsic HAT activity a lysine cluster located in the hinge region of the GR (A) through which Clock reduces affinity of GR to its cognate DNA GREs (B). A: acetylation; Bmal1: brain-muscle-arnt-like protein 1; DBD: DNA-binding domain; GREs: glucocorticoid response elements; HR: hinge region; K: lysine